Journal: Cell Death & Disease

Article Title: PHF23 promotes NSCLC proliferation, metastasis, and chemoresistance via stabilization of ACTN4 and activation of the ERK pathway

doi: 10.1038/s41419-023-06069-4

Figure Lengend Snippet: Antibodies for western blot.

Article Snippet: Phospho-JUN (Ser73) , Proteintech , 28891-1-AP , Rabbit , 1:1000.

Techniques: Western Blot

Journal: Frontiers in Physiology

Article Title: RARRES1 attenuates H 2 O 2 -induced RPE cell injury and inhibits choroidal neovascularization

doi: 10.3389/fphys.2025.1641653

Figure Lengend Snippet: Mechanistic Role of RARRES1 in ARPE-19 Cells Under Oxidative Stress. (A) Western blot analysis revealed that knockdown of RARRES1 in ARPE-19 cells under oxidative stress conditions led to activation of phosphorylated JNK (p-JNK) and suppression of Nrf2 and SIRT1 expression. (B) In contrast, RARRES1 overexpression reversed these effects, suggesting a protective regulatory mechanism. Protein levels were normalized to β-actin, and mRNA levels to GAPDH. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ns = not significant.

Article Snippet: The primary antibodies include RARRES1 (sc-390461, Santa Cruz), phospho-JNK (HY- P80831 , MCE), JNK (HY- P80197 , MCE), phospho-ERK (HY- P81156 , MCE), ERK (HY- P80393 , MCE), Nrf-2 (80593-1-RR, Proteintech), SIRT-1 (HY- P80319 , MCE), Occludin (502601, ZEN), β-actin (HRP-66009, Proteintech).

Techniques: Western Blot, Knockdown, Activation Assay, Expressing, Over Expression

Journal: Neurochemical Research

Article Title: Exogenous TIPE2 Inhibit TAK1 to Improve Inflammation and Neuropathic Pain Induced by Sciatic Nerve Injury Through Inactivating NF-κB and JNK

doi: 10.1007/s11064-022-03671-4

Figure Lengend Snippet: TIPE2 inhibited activation of NF-κB and JNK via downregulation of TAK1. A Immunofluorescence staining was performed to analyze phosphor-JNK in spinal cord and DRG after 14 days. Mean fluorescence intensity of phospho-JNK was analyzed by Image J 1.49p. The scale bar was 20 μm. B Western blot was carried out to analyze the expression of p-NF-κB p65, p-JNK1, and p-JNK2 in spinal cord after 14 days. && p < 0.01 versus Sham; % p < 0.05, %% p < 0.01 versus Model; $ p < 0.05, $$ p < 0.01 versus TIPE2-1; ! p < 0.05, !! p < 0.01 versus TIPE2-5

Article Snippet: After blocking by 5% non-fat milk, the membranes were incubated with primary antibodies against p-IκBα (1:10000, ab133462, Abcam), IκBα (1:800, ab95338, Abcam), p-JNK1(1:1200, ab47337, Abcam), p-JNK2 (1:1000, P02706, Boster), JNK1 (1:2500, ab199380, Abcam), JNK2 (1:1500, 51153-1-AP, Proteintech), β-actin (1:2000, 20536-1-AP, Proteintech), TIPE2 (1:1000, orb158628, Biorbyt), TAK1 (1:800, orb256677, Biorbyt) at 4 °C overnight.

Techniques: Activation Assay, Immunofluorescence, Staining, Fluorescence, Western Blot, Expressing